The Karbstein Lab in the Department of Biochemistry at Vanderbilt University is seeking an enthusiastic molecular biology/biochemistry research technician to aid in the lab's studies of ribosome assembly, quality control and repair. In addition to providing a better understanding about the cellular mechanisms underlying ribosome integrity, this basic science work is highly relevant to cancer biology, and the biology of aging. The lab relies heavily on biochemistry and molecular biology, as well as yeast genetics. For examples of the type of research that will be undertaken see: Blomqvist et al, 2023, PLoS Genetics; Yang et al, 2023, Mol Cell; and Parker et al, 2024, PLoS Biology.

The lab is a mid-sized lab seeking a research tech who will spend most of their time performing research, with some time spent maintaining lab infrastructure. The new research tech will be directly mentored and instructed by Dr. Karbstein, the Principle Investigator, to learn how to plan, conduct, interpret and present research. The laboratory is a supportive, fast-paced environment for driven candidates who enjoy a collaborative atmosphere. It is expected that the new research tech will produce enough data for a first author publication within 2 years of joining the lab. We have a strong post-baccalaureate training record: our past two research techs each produced enough data for a first author publication and were admitted to top PhD programs in the biomedical sciences. The lab strives to be a diverse, inclusive, and equitable working environment; prioritizes personal growth and wellness; and is committed to work-life balance. Overall, this is an excellent opportunity for any recent college graduate looking to obtain research experience before applying to graduate school or medical school.

Duties and Responsibilities:

Assists in Research and Development:

• Uses protein gels and Western blotting to analyze ribosome assembly factors and ribosomal proteins

• Uses Northern blotting to analyze ribosome assembly in cells

• Uses agarose gels to analyze PCR screens and PCR reactions

• Generates yeast strains

• Uses quantitative growth measurements to monitor genetic interactions

• Designs and clones proteins and RNAs for yeast in vivo work and in vitro assays

• Expresses and purifies proteins in bacteria

Performs general lab and administrative duties:

• Prepare buffers and reagents.

• Order supplies

• Receive packages

• Other tasks as directed by supervisor

Qualifications

• A Bachelor’s degree in cellular biology, molecular biology, biochemistry, or a related field from an accredited institution of higher education is necessary.

• Ability to perform all basic calculations related to buffer composition (Molarity, dilution factor, etc.) is necessary.

• Being comfortable in learning new assays is necessary.

Commitment to Equity, Diversity, and Inclusion

At Vanderbilt University, we are intentional about and assume accountability for fostering advancement and respect for equity, diversity, and inclusion for all students, faculty, and staff. Our commitment to diversity makes us who we are. We have created a community that celebrates differences and lets individuality thrive. As part of this commitment, we actively value diversity in our workplace and learning environments as we seek to take advantage of the rich backgrounds and abilities of everyone. The diverse voices of Vanderbilt represent an invaluable resource for the University in its efforts to fulfill its mission and strive to be an example of excellence in higher education.

Vanderbilt University is an equal-opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran, or any other characteristic protected by law.

Please note, all candidates selected for an offer of employment are subject to pre-employment background checks, which may include but are not limited to, based on the role for which they have been selected: criminal history, education verification, social media review, motor vehicle records, credit history, and professional license verification.

Commitment to Equity, Diversity, and Inclusion At Vanderbilt University, we are intentional about and assume accountability for fostering advancement and respect for equity, diversity, and inclusion for all students, faculty, and staff. Our commitment to diversity makes us who we are. We have created a community that celebrates differences and lets individuality thrive. As part of this commitment, we actively value diversity in our workplace and learning environments as we seek to take advantage of the rich backgrounds and abilities of everyone. The diverse voices of Vanderbilt represent an invaluable resource for the University in its efforts to fulfill its mission and strive to be an example of excellence in higher education. Vanderbilt University is an equal opportunity, affirmative action employer. Women, minorities, people with disabilities, and protected veterans are encouraged to apply.